PT-141 is supplied as a lyophilised powder. This step-by-step guide covers accurate reconstitution, concentration calculation, and correct storage for research use.
PT-141 (Bremelanotide) — research reconstitution guide
PT-141, a synthetic analogue of the peptide alpha-MSH, is supplied by My UK Peptides as a lyophilised (freeze-dried) powder in sealed glass vials. Lyophilisation preserves the compound during storage and transit; reconstitution is required before it can be used in aqueous-phase laboratory work.
This guide provides a step-by-step reconstitution protocol for research purposes only.
Required materials
- PT-141 vial (lyophilised powder)
- Bacteriostatic water (preferred reconstitution solvent) or sterile water
- 1 ml sterile insulin syringes or transfer needles
- Alcohol swabs (isopropyl alcohol 70%)
- Gloves and appropriate PPE
Before you begin
Ensure your work area is clean and prepared. Wash hands thoroughly and apply nitrile gloves. Allow both the PT-141 vial and the bacteriostatic water to reach room temperature if taken from cold storage — this prevents pressure differentials during needle insertion.
Step-by-step reconstitution
- Prepare the vials. Remove the flip-off caps from both the peptide vial and the bacteriostatic water. Wipe both rubber stoppers with an alcohol swab and allow to dry.
- Equalise vial pressure. Insert a needle briefly into the peptide vial to equalise any internal vacuum. You may notice air being drawn in or expelled — this is normal.
- Add bacteriostatic water. Draw up the desired volume of bacteriostatic water (see concentration table below). Insert the needle at a 45° angle and allow the liquid to run gently down the inside wall of the vial — do not inject directly onto the lyophilised puck, as this can degrade the peptide.
- Allow to dissolve. Gently roll the vial between your fingers. Do not shake vigorously. Allow 2–3 minutes for full dissolution. The solution should become clear.
Concentration calculations
| Water added (ml) | Concentration per 0.1 ml (10 units) |
|---|---|
| 1 ml | 500 mcg |
| 2 ml | 250 mcg |
| 3 ml | 167 mcg |
Storage after reconstitution
Store reconstituted PT-141 in the refrigerator (2–8°C). When stored correctly in bacteriostatic water, it should remain stable for up to 28 days. Do not freeze a reconstituted solution. Keep the vial sealed, away from light, and record the date of reconstitution on the label.
Research use only. This guide is for informational and laboratory purposes only. PT-141 from My UK Peptides is not for human or veterinary use.